Technical Data
Media
Skim Milk Agar (Plate Count)
S1013-90M
Microbiological Grade
Storage 4°C    Shipping RT
Formulation (g/Liter):
Agar10.5
Glucose1.0
Skim Milk powder (no inhibitors)1.0
Casein Peptone5.0
Yeast Extract2.5
Total:20g/Liter
For determining the microbial count in milk and dairy products. The culture medium complies with the recommendations of the International Dairy Federation (Internationaler Milchwirtschafts-verband) (1991) and the DIN Norm 10192 for the examination of milk and dairy products.

Addition of skim milk to a culture medium with a superior nutrient base optimally adapts it to the neutral conditions experienced by microorganisms which grow in milk. More colonies grow and a wider range of bacteria can be cultured than in other culture media intended for the same purpose (TERPLAN et al. 1967).

Appearance:
Off-white to tan, homogeneous, free flowing powder
Solubility:
Colorless to yellow, clear to opalescent, complete after autoclaving.
pH:
As Reported


Directions per Liter: Suspend 20.0 g/liter in cold water and allow to stand for about 15 minutes. Transfer flask to a cold water bath and heat gently, with frequent shaking, until completely dissolved, then autoclave (15 min at 121°C).

Storage and Stability: Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Formulation (g/Liter): Agar 10.5 Glucose 1.0 Skim Milk powder (no inhibitors) 1.0 Casein Peptone 5.0 Yeast Extract 2.5

Total: 20g/Liter

Directions per Liter: Suspend 20.0 g/liter in cold water and allow to stand for about 15 minutes. Transfer flask to a cold water bath and heat gently, with frequent shaking, until completely dissolved, then autoclave (15 min at 121°C).

Storage and Stability: Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

DIN Deutsches Institut für Normung e.V.: Mikrobiologische Milchuntersuchung; Bestimmung der Keimzahl (Referenzverfahren). - DIN 10192.
Internationaler Milchwirtschaftsverband: Milch u. Milchprodukte, Zählung von Mikroorganismen (Koloniezählung bei 30°C) - Internationaler Standard 100 (1991).
Internationaler Milchwirtschaftsverband: Flüssige Milch. Zählung von psychotrophen Mikroorganismen (Koloniezählung bei 6,5°C). - Internationaler Standard 101 (1991).
TERPLAN, G., RUNDFELDT, H. u. ZAADHOF, K.-J.: Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967).