Technical Data
Skim Milk Agar (Plate Count)
Molecular Biology Storage: 4°CShipping: RT
For determining the microbial count in milk and dairy products. The culture medium complies with the recommendations of the International Dairy Federation (Internationaler Milchwirtschafts-verband) (1991) and the DIN Norm 10192 for the examination of milk and dairy products.

Addition of skim milk to a culture medium with a superior nutrient base optimally adapts it to the neutral conditions experienced by microorganisms which grow in milk. More colonies grow and a wider range of bacteria can be cultured than in other culture media intended for the same purpose (TERPLAN et al. 1967).

Off-white to tan, homogeneous, free flowing powder
Colorless to yellow, clear to opalescent, complete after autoclaving.
As Reported
Formulation (g/Liter):
Agar 10.5
Glucose 1.0
Skim Milk powder (no inhibitors) 1.0
Casein Peptone 5.0
Yeast Extract 2.5

Total: 20g/Liter

Directions per Liter:
Suspend 20.0 g/liter in cold water and allow to stand for about 15 minutes. Transfer flask to a cold water bath and heat gently, with frequent shaking, until completely dissolved, then autoclave (15 min at 121°C).

Storage and Stability:
Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
DIN Deutsches Institut für Normung e.V.: Mikrobiologische Milchuntersuchung; Bestimmung der Keimzahl (Referenzverfahren). - DIN 10192.
Internationaler Milchwirtschaftsverband: Milch u. Milchprodukte, Zählung von Mikroorganismen (Koloniezählung bei 30°C) - Internationaler Standard 100 (1991).
Internationaler Milchwirtschaftsverband: Flüssige Milch. Zählung von psychotrophen Mikroorganismen (Koloniezählung bei 6,5°C). - Internationaler Standard 101 (1991).
TERPLAN, G., RUNDFELDT, H. u. ZAADHOF, K.-J.: Zur Eignung verschiedener Nährböden für die Bestimmung der Gesamtkeimzahl der Milch. - Arch. Lebensmittelhyg., 18; 9-11 (1967).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.