Technical Data
S1014-15D
Skp1 (S-phase Kinase-associated Protein, p19skp1)
Description:
SKP1 (S-phase kinase-associated protein 1) is a core component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase protein complex. It mediates the ubiquitination of proteins involved in cell cycle progression, transcriptional regulation, and signal transduction. Among the components of the SCF complexes, SKP1 is an adaptor between one of the variable F-box proteins and Cullin (1). Independent of its function as a part of the SCF complex, SKP1 also functions in mitotic exit and cytokinesis. A possible contribution to the function of SKP1 in the mitotic exit is its physical interaction with Bfa1, a key negative regulator of the mitotic exit network (2). SKP1 also plays a crucial role in proper chromosomal segregation, which is required for the maintenance of euploidy and suppression of transformation (3).
Applications:
Suitable for use in Western Blot, Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000-1:10,000
Flow cytometry: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E549Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on human SKP1.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human SKP1. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kong H, et al. Mol. Biol. Evol. 21(1):117-128, 2004 2. Kim N, et al. The Journal of Microbiology 44(6):641-648, 2006 3. Piva R, et al. Molecular and Cellular Biology 22(23):8375- 8387, 2002