Technical Data
S1014-20B
SLP-76 (lymphocyte cytosolic protein 2, SH2 domain containing leukocyte protein of 76kD, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76kD, SLP76, SLP-76 tyrosine phosphoprotein)
Description:
Detects SLP-76 (SH2 domain containing Leukocyte Protein) of 76kD, a hematopoietic cell-specific adapter protein which intereacts with other signaling molecules through amino-terminal phosphotyrosine residues, a central proline-rich region and a carboxyl-terminal Src homology 2 domain (SH2).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
RAW264 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9H275Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Monoclonal antibody from BALB/c-derived hybridoma. Cellular Localization: Cytoplasm (Probable).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in sterile filtered PBS, 0.1% sodium azide.
Specificity:
Recognizes human SH2 (Src homology 2) domain-Leukocyte Protein of 76kD. Species Crossreactivity: mouse. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wardenburg, J.B., et al. (1996). J. Biol. Chem. 271: 19641-19644. 2. Jackman, K. J., et al. (1995). J. Biol. Chem. 270: 7029-7032.