		Technical Data
S1014-20B	SLP-76 (lymphocyte cytosolic protein 2, SH2 domain containing leukocyte protein of 76kD, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76kD, SLP76, SLP-76 tyrosine phosphoprotein)

Description:
Detects SLP-76 (SH2 domain containing Leukocyte Protein) of 76kD, a hematopoietic cell-specific adapter protein which intereacts with other signaling molecules through amino-terminal phosphotyrosine residues, a central proline-rich region and a carboxyl-terminal Src homology 2 domain (SH2).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
RAW264 cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	9H275	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Monoclonal antibody from BALB/c-derived hybridoma. Cellular Localization: Cytoplasm (Probable).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in sterile filtered PBS, 0.1% sodium azide.

Specificity:

Recognizes human SH2 (Src homology 2) domain-Leukocyte Protein of 76kD. Species Crossreactivity: mouse. Species sequence homology: rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wardenburg, J.B., et al. (1996). J. Biol. Chem. 271: 19641-19644. 2. Jackman, K. J., et al. (1995). J. Biol. Chem. 270: 7029-7032.