Technical Data
SLP-76 (lymphocyte cytosolic protein 2, SH2 domain containing leukocyte protein of 76kD, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76kD, SLP76, SLP-76 tyrosine phosphoprotein)
SLP-76 is an adapter protein which is rapidly tyrosine phosphorylated following ligation of the T-cell Receptor. SLP 76 has an SH2 domain, a proline-rich region, and numerous putative tyrosine phosphorylation sites. In addition, SLP-76 binds GRB2 through a 20 amino acid stretch in the proline-rich region of SLP76. Overexpression of SLP 76 results in greatly augmented IL-2 production. SLP-76 therefore appears to play an important role in the signaling pathways involved in T-cell activation.
Detects SLP-76 (SH2 domain containing Leukocyte Protein) of 76kD, a hematopoietic cell-specific adapter protein which intereacts with other signaling molecules through amino-terminal phosphotyrosine residues, a central proline-rich region and a carboxyl-terminal Src homology 2 domain (SH2).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000 detected SLP-76 on 10ug of RAW 264 lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
RAW264 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a9H275Affinity Purified
100ug-20CBlue IceHumanMouse
BALB/c-derived hybridoma (NM_005565.3). Cellular Localization: Cytoplasm (Probable).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.08% sodium azide.
Recognizes human SH2 (Src homology 2) domain-Leukocyte Protein of ~76kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wardenburg, J.B., et al. (1996). J. Biol. Chem. 271: 19641-19644. 2. Jackman, K. J., et al. (1995). J. Biol. Chem. 270: 7029-7032.