Technical Data
S1014-20L
SLP 76, phosphorylated (Tyr145) (SH2-domain Leukocyte Protein)
Description:
The activation of protein tyrosine kinases is a critical event in T cell antigen receptor (TCR)-mediated signaling. One substrate of the TCR-activated protein tyrosine kinase pathway is a 76-kD protein (SLP-76) that associates with the adaptor protein Grb2. Northern analysis demonstrates that SLP76 mRNA is expressed solely in peripheral blood leukocytes, thymus, and spleen; and in human T cell, B cell and monocytic cell lines. In vitro translation of SLP76 cDNA gives rise to a single product of 76kD that associates with a GST/Grb2 fusion protein, demonstrating a direct association between these two molecules. It has been reported that this novel protein is likely to play an important role in TCR-mediated intracellular signal transduction (1). It has been shown that SDF-1alpha stimulation of prolonged ERK activation in Jurkat T cells requires both the ZAP-70 tyrosine kinase and the Src homology 2 domain-containing leukocyte protein of SLP-76 scaffold protein. This pathway involves ZAP-70-dependent tyrosine phosphorylation of SLP-76 at one or more of its tyrosines, 113, 128, and 145 (2).

Applications:
Suitable for use inFlow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100,000-1:200,000
Immunocytochemistry: 1:100
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9E550Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A phospho specific peptide corresponding to residues surrounding tyrosine 145 of human SLP-76.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human SLP-76 phosphorylated at Tyr145.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jackman JK, et al J Biol Chem 270(13):7029-32, 1995 2. Kremer KN, et al. J Immunol. 17(1):360-7, 2003