Technical Data
S1014-20R
SLP-76 (LCP2, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) Lymphocyte cytosolic protein 2, SH2 domain-containing leucocyte protein of 76kD, SH2 domain-containing leukocyte protein of 76kD,SLP-76 tyrosine phosphoprotein
Description:
SLP-76 is a 533aa, 76kD tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC , ITK, and
ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation.
Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76,
respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19H180Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~500ug/ml
Immunogen:
Recombinant human PP4 aa1-533; Q13094 (E. coli).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Specificity:
Recognizes human SLP-76 at ~90kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.