		Technical Data
S1014-20R	SLP-76 (LCP2, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) Lymphocyte cytosolic protein 2, SH2 domain-containing leucocyte protein of 76kD, SH2 domain-containing leukocyte protein of 76kD,SLP-76 tyrosine phosphoprotein

Description:
SLP-76 is a 533aa, 76kD tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC , ITK, and
ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation.
Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76,
respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9H180	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~500ug/ml

Immunogen:

Recombinant human PP4 aa1-533; Q13094 (E. coli).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.

Specificity:

Recognizes human SLP-76 at ~90kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.