Technical Data
Sm (Smith) Antigen
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Suitable for use in ELISA and Immunodiffusion. Other applications not tested.

Recommended Dilution:
ELISA: 1:100
Optimal dilutions to be determined by the researcher.

Immunodiffusion: 1:2

Other Autoantibody Activity in the Serum:
The serum exhibits no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, SCL, Jo-1, mitochondrial or ribosomal antigens in an ELISA system designed to detect IgG antibody subclasses.

Interpretation of Results: <16EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20EU/ml:
SSA: 3EU/ml
SSB: 3EU/ml
SCL: 4EU/ml
Sm: 695EU/ml
Sm/RNP: 220EU/ml
Jo-1: 5EU/ml

Interpretation of Results:
Negative: <16EU/ml, Equivocal: 16-20EU/ml, Positive: >20EU/ml

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
2ml-20CBlue IceHumanHuman
Not Determined
Sm Antigen. Autoantibody derived from human serum.
Purified by fractionation of delipidated and defibrinated donor human serum.
Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Recognizes human Sm antigen. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. Anti-RNP precipitant is detected. This product shows no reactivity at a dilution of 1:100 when tested against purified La/SS-B, Scl-70, Jo-1, Mitochondrial or Ribosomal P antigens in an ELISA system designed to detect IgG antibody. Anti-RNP and low levels of anti-SSA activity are detected. Species Crossreactivity: rabbit
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.