Technical Data
S1014-29B
Sm (Smith) Antigen
Description:
The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Applications:
Suitable for use in Affinity Purification, Electron Microscopy, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1.0ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minute at RT. No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control: LS174T cells. Tonsil.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20C. Aliquots are stable for at least 12 months at-20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG3,k3F332Affinity Purified
SizeStorageShippingSourceHost
500ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Sm (Smith) Antigen. MW of Antigen: ~25kD (predominan). Cellular Localization: Nuclear.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See S1014-29BX
Specificity:
Recognizes cross-reactive epitopes on the B/B and D polypeptides of Sm. Precipitates the small nuclear RNAs U1, U2, U4, U5 and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes. Species Crossreactivity: Human, porcine, rabbit, rat, Drosophila.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lerner EA; et al. Proc Natl Acad Sci USA, 1981, 78(5):273741. 2. James JA; et al. Clinical and Experimental Immunology, 1994 Dec, 98(3):41926. 3. Kiseleva E, et al.. Embo Journal, 1994, 13(24):6052- 61. 4. Moussa F; et al. Cell and Tissue Research, 1994 Nov, 278(2):36378
Additional: 1. Puvion-Dutilleul F; et al. Journal of Cell Science, 1994 Jun, 107 ( Pt 6):145768. 2. Pyne CK; et al. Chromosoma, 1994 Dec, 103(7):47585. 3. Hirakata M; et al. Journal of Immunology, 1993, 150:3592601. 4. Prather RS; et al. Molecular Reprod and Development, 1992, 33(2):11923. 5. Segalat L; et al. Biol of the Cell, 1992, 75(3):1815. 6. Eisenberg RA; et al. J Clin Invest, 1990, 85(1):8692.