		Technical Data
S1014-50F	SMAD1, phosphorylated (Ser206) (Mothers Against Decapentaplegic Homolog 1, Mothers Against DPP Homolog 1, MAD Homolog 1, MADH1, SMAD Family Member 1, SMAD 1, hSMAD1, BSP-1, BSP1, JV4-1, JV41, Mad-related Protein 1, MADR1, Transforming Growth Factor-beta-s

Description:
Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-b family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5).MAP kinases and CDKs 8 and 9 phosphorylate residues in the linker region of Smad1, including Ser206. The phosphorylation of Ser206 recruits Smurf1 to the linker region and leads to the degradation of Smad1 (6). Phosphorylation of this site also promotes Smad1 transcriptional action by recruiting YAP to the linker region (7).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	D40B7	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic phosphopeptide corresponding to residues surrounding Ser206 of human Smad1.

Purity:

Supernatant

Form

Supplied as a liquid in 10M sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Specificity:

Recognizes endogenous levels of human Smad1, phosphorylated at Ser206. Species Crossreactivity: Monkey, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Hogan, B.L. et al. (1996) Genes Dev. 10, 1580-1594. 2.Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3.Klemm, J.D. et al. (1998) Annu. Rev. Immunol. 16, 569-592. 4.Kretzschmar, M. et al. (1997) Genes Dev. 11, 984-995. 5.Whitman, M. (1998) Genes Dev. 12, 2445-2462. 6.Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7.Alarcón, C. et al. (2009) Cell 139, 757-69.