		Technical Data
S1014-51G	Smad1 (SMAD Family Member 1, SMAD-1, BSP1, BSP-1, Dwarfin A, DwfA, hSMAD1, hSMAD-1, JV41, JV4-1, MAD Mothers Against Decapentaplegic Homolog 1, Mothers Against Decapentaplegic Homolog 1, Mothers Against DPP Homolog 1, MADH1, Mad-related Protein 1, MADR1,

Description:
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-beta and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to amino acid residues around 253 of human Smad1.

Purity:

Purified by immunoaffinity chromatography.

Form

phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA

Specificity:

The antibody recognizes ~60kD human Smad1. Reactivity to other species has not been tested.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.