Technical Data
S1014-52E2
SMAD2 (MADH2, JV18, MADR2, SMAD2, JV18-1, MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 2, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 2)
Description:
SMAD2 protein (also known as MADH2) is a transcriptional modulator activated by TGF beta. It is found at high levels in skeletal muscle, heart and placenta. SMAD2 exists in isoforms, a predominant long form and a short form missing the 30aa exon 3. The short form shows DNA binding and higher transcriptional activation activity. SMAD2 may act as a tumor suppressor in colorectal carcinoma.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:1,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Smad2. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.