		Technical Data
S1014-52E3	SMAD2 (MADH2, JV18, MADR2, SMAD2, JV18-1, MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 2, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 2)

Description:
SMAD2 protein (also known as MADH2) is a transcriptional modulator activated by TGF beta. It is found at high levels in skeletal muscle, heart and placenta. SMAD2 exists in isoforms, a predominant long form and a short form missing the 30aa exon 3. The short form shows DNA binding and higher transcriptional activation activity. SMAD2 may act as a tumor suppressor in colorectal carcinoma.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic non-phosphopeptide corresponding to human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes human Smad2. Species Cross-Reactivity: mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.