Technical Data
S1014-53H1
Smad3 (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Description:
Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 2.5ug/ml
Western Blot: 1:2000-1:20,000
ELISA: 1:25,000-1:100,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide, KLH-conjugated. Epitope: Amino acids 417-425 of human SMAD3 protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes Human SMAD, Mothers Against DPP Homolog 3 (SMAD3). Species Crossreactivity: Bovine, Chicken, Frog, Human, Mouse, Pig, Rat, Zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.