		Technical Data
S1014-55C1	SMAD4, phosphorylated (Thr277) (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4)

Description:
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Applications:
Suitable for use in ELISA, Dot Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Immunohistochemistry: paraffin
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr277 of human SMAD4 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human SMAD4 when phosphorylated at Thr277.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004). 2.Horvath, L.G., et al., Prostate 59(3):234-242 (2004). 3.Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004). 4.Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004). 5.Maru, D., et al., Oncogene 23(3):859-864 (2004).