Technical Data
S1014-55C2
SMAD4 (T277) (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4)
Description:
SMAD4 is the common SMAD (co-SMAD) mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunofluorescence :1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to amino acid residues surrounding S277 of human SMAD4 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human SMAD4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004). 2.Horvath, L.G., et al., Prostate 59(3):234-242 (2004). 3.Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004). 4.Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004). 5.Maru, D., et al., Oncogene 23(3):859-864 (2004).