Technical Data
SMAD 1/5/8/9 (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma)
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Positive control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptides corresponding to aa19-33 and 315-329 of human Smad1. Sequence homology: Smad1, Smad5, Smad8, Smad9.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes human Smad5. Crossreactivity: Smad1, Smad8, Smad9. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gemma,A., Hagiwara,K., Vincent,F., Ke,Y., Hancock,A.R., Nagashima,M., Bennett,W.P. and Harris,C.C. hSmad5 gene, a human hSmad family member: its full length cDNA genomic structure, promoter region and mutation analysis in human tumors. Oncogene 16 (7), 951-956 (1998)