Technical Data
S1014-55H3
SMAD6 (Mothers Against decapentaplegic homolog 6, MAD Homolog 6, mothers against DPP Homolog 6, SMAD Family Member 6, SMAD 6, hSMAD6, MADH6)
Description:
Signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily are known to be mediated by SMAD proteins. Biological activities mediated by SMADs include cell growth and morphogenesis, development, and immune response. Activation by TGF-b is mediated by Smad2 and Smad3 while activation by bone morphogenetic protein (BMP) is mediated by Smad 1 and Smad 5. In contrast to the activating effects of these Smad proteins, Smad6 and Smad7, whose expression is also induced by ligand binding, appear to function as negative regulators of TGF-b superfamily signaling pathways.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 5ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa85-99 and amino acids 372-388 (NLEQRSESVRRTRSKIG) of human SMAD6 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human SMAD6. Species sequence homology: Monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.