Technical Data
S1014-73C
SNAP 25 (Synaptosomal-associated Protein 25, Synaptosomal Associated Protein 25, Synaptosomal-associated 25kD Protein, Synaptosomal-associated Protein 25kDa, SNAP25, SNAP-25, bA416N4.2, dJ1068F16.2, FLJ23079, HGNC:11132, Resistance to Inhibitors of Cholin
Description:
SNAP 25 (synaptosome-associated protein of 25kD) is a SNAP (soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein) receptor (SNARE-related protein) which is tightly associated with the plasma membrane by means of the palmitoylation of a cluster of cysteine residues with its structure. SNAP 25 is specifically cleaved close to the carboxyl terminal by the botulinum neurotoxins (BoNT) A and E which are known to inhibit neurotransmitter release. Similar inhibitory effects of BoNT/A and E have been recorded for non-neuronal secretory cells, such as adrenal medulla, This leads to the identification of SNAP 25 in SNARE-like complexes precipitated from chromaffin cells.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:5000. Detects a band at ~26-27kD.
Immunohistochemistry (Paraffin): 1:2000-1:5000. No pretreatment or antigen retrieval using heat treatment is required.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19E717Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Crude human synaptic immunoprecipitate. Cellular Localization: Cytoplasmic and plasma membrane.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human SNAP 25. Species Crossreactivity: rat and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honer, W.G., et al., Hippocampal synaptic pathology in patients with temporal lobe epilepsy. Acta Neuropathol (Berl) 87: 202-210 (1994). 2. Honer, W.G., et al., Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609: 9-20 (1993). 3. Honer, W.G., et al., Regional synaptic pathology in Alzheimer's disease. Neurobiol. Aging 13: 375-382 (1992). 4. Honer, W.G., et al., Characterization of a synaptic antigen of interest in neuropsychiatric illness. Biol Psychiatry 31: 147-158 (1992).