Technical Data
S1040-01J
SOCS 1 (Suppressor of Cytokine Signaling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1, Cish1, Ssi1)
Description:
Suppressor of cytokine signaling 1 (SOCS1), also known as Jak-binding protein (JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211aa protein has a molecular mass of about 23.5kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13.2 SOCS1 regulates the JAK/STAT signal-transduction pathway. Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1-2ug/ml
Immunohistochemistry (paraffin): 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequence.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity:
Recognizes human SOCS1. Species Cross-Reactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.