		Technical Data
S1040-01K	SOCS 1 (Suppressor of Cytokine Signalling 1, SOCS1, SOCS-1, Cytokine Inducible SH2 Protein 1, CISH1, Janus Kinase Binding Protein, JAK-binding Protein, JAB, STAT-induced STAT Inhibitor 1, SSI1, SSI-1, TEC-interacting Protein 3, TIP3, TIP-3)

Description:
The Suppressor of Cytokine Signaling (SOCS) family of proteins acts in a classic negative feedback loop to regulate cytokine signal transduction. SOCS-1, also designated Janus kinase [JAK] binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is a 24kD protein containing an SH2 domain. SOCS-1 inhibits both IL-6-induced receptor phosphorylation and STAT activation. SOCS-1 is also 45 induced by and inhibits the signaling action of IL-3, IL-4, 1 2 3 4kD 97 66 LLIF, prolactin, and erythropoietin. Additionally, SOCS-1 can associate with all 4 JAK family kinases, and is an important regulator of the JAK-STAT signaling pathway. SOCS-1 is found primarily in thymocytes, where it plays a key role in the negative regulation of IFN-g signaling and T cell differentiation.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Immunoprecipitation: 10ug/200ul of cell extract from 5x10e6 cells.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 with C57BL/6 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11n174	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant corresponding to aa2-78 from mouse GST-SOCS-1.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 100ul PBS, pH 7.2, 50% glycerol.

Specificity:

Recognizes mouse SOCS-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1) Zhang, J. G., et al. Proc. Natl. Acad. Sci. USA, 98,13261-13265 (2001) 2) Stoiber, D., et al. J. Immunol. 166, 466-472 (2001) 3) Croker, B. A., et al. Nat. Immunol. 4, 540-545 (2003) Clone 4H1 is used in reference number.