Technical Data
S1040-02M
SOCS2, ID (Suppressor of Cytokine Signaling 2, SOCS-2, Cytokine-inducible SH2 Protein 2, CIS-2, STAT-induced STAT Inhibitor 2, SSI-2, CIS2, SSI2, STATI2)
Description:
Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous aa long sequence at the C-terminus that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-2 is 198aa long with short N-terminus and shares sequence similarity with CIS. SOCS-2 mRNA normally presents at very low levels in different tissues; however, its level is rapidly increased upon stimulation with IL-1, IL-3, IL-4, IL-6, LIF, GM-CSF, G-CSF, EPO, IFNg, prolactin, and GH. Similar to CIS, SOCS-2 does not bind to Janus- activated kinases; however, it can associate with GH, IGF-1, prolactin and leptin receptors. For example, SOCS-2 binds to Y1077 motif of leptin receptor and thus acts as a negative regulator of STAT5.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to the intermediate region of human SOCS-2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes human SOCS-2. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hilton D. J. et al. (1998) Biochemistry 195: 114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15 (11): 1493-1504. 4. Cooney R.N. (2002) Shock 17(2): 83-90. 5. Lavens D. (2006) J. of Cell Sc. 119: 2214-2224. 6. GoldshmitGoldshmit Y. et al. (2004) J. of Biol. Chem. 279(16):16349-16355.