		Technical Data
S1040-02M	SOCS2, ID (Suppressor of Cytokine Signaling 2, SOCS-2, Cytokine-inducible SH2 Protein 2, CIS-2, STAT-induced STAT Inhibitor 2, SSI-2, CIS2, SSI2, STATI2)

Description:
Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous aa long sequence at the C-terminus that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-2 is 198aa long with short N-terminus and shares sequence similarity with CIS. SOCS-2 mRNA normally presents at very low levels in different tissues; however, its level is rapidly increased upon stimulation with IL-1, IL-3, IL-4, IL-6, LIF, GM-CSF, G-CSF, EPO, IFNg, prolactin, and GH. Similar to CIS, SOCS-2 does not bind to Janus- activated kinases; however, it can associate with GH, IGF-1, prolactin and leptin receptors. For example, SOCS-2 binds to Y1077 motif of leptin receptor and thus acts as a negative regulator of STAT5.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Synthetic peptide corresponding to the intermediate region of human SOCS-2.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Specificity:

Recognizes human SOCS-2. Species Crossreactivity: rat and mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hilton D. J. et al. (1998) Biochemistry 195: 114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15 (11): 1493-1504. 4. Cooney R.N. (2002) Shock 17(2): 83-90. 5. Lavens D. (2006) J. of Cell Sc. 119: 2214-2224. 6. GoldshmitGoldshmit Y. et al. (2004) J. of Biol. Chem. 279(16):16349-16355.