		Technical Data
S1040-03T	SOCS3, Induced (Suppressor of Cytokine Signaling 3, SOCS-3, Cytokine-inducible SH2 Protein 3, CIS-3, SSI-3, CIS3, SSI3, STAT-induced STAT Inhibitor 3)

Description:
Suppressors of cytokine signaling (SOCS) protein family include eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-3 is 225aa long and shares sequence similarity with SOCS- 1. Its expression in many tissues is induced by elevated levels of IL-1, IL-4, IL-6, LIF, GM-CSF, IFNg, GH, EPO, leptin, prolactin, and other cytokines. However, SOCS-3 is found mostly in lungs, spleen, and fat. SOCS-3 targets Janus activated kinases similar to SOCS-1, but with lower affinity and does not block their activity. In addition, SOCS-3 binds phosphorylated tyrosine (Y) residues of various receptors: Y759 of gp130, Y333/338 of GHR, and Y985,1077 of leptin receptor.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200-1:500
Immunohistochemistry: 1:100-1:200
Immunoprecipitation: 1:50-1:200 extract from 10e7 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Synthetic peptide corresponding to the C-terminus of human SOCS-3.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 50% glycerol.

Specificity:

Recognizes human SOCS-3. Species sequence homology: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. HiltonD.J.etal.(1998)Biochemistry 195:114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15(11):1493-1504. 4. Cooney R. N. (2002) Shock 17 (2):83-90. 5. Greenhalgh C.J. et al. (2001) J. of Leuk. Biol. 70: 348-356. 6. Auernhammer C. J. et al. (2001) J. Clin. Invest. 108: 1735-174.