Technical Data
SOCS3, ID (Suppressor of Cytokine Signaling 3, SOCS-3, Cytokine-inducible SH2 Protein 3, CIS-3, SSI-3, CIS3, SSI3, STAT-induced STAT Inhibitor 3)
Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and aa composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT- induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:S1040-03R

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the internal sequence of human SOCS-3.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes human SOCS-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gregorieff A et al. (2000) J. Biol. Chem. 275(28): 21596-21604. 2. Krebs DL and Hilton DJ (2000) J. Cell Sci. 113:2813-2819 3. Ram PA and Waxman (1999) J. Biol. Chem. 274(50): 35553-61. 4. Hilton DJ et al (1998) Proc. Natl. Acad. Sci USA 95: 114-119 5. Yoshimura A (1998) Cytokine Growth Factor Rev 9: 197-204 6. Minamoto S et al (1997) Biochem Biophys Res Commun 237: 79-83 7. Cohney SJ et al (1999) Mol. Cell Biol. 19: 4980-88. 8. Davey HW et al (1999) Mol. Cwll Endocrinol 158: 111-16.