Technical Data
SOCS4, NT (Suppressor of Cytokine Signaling 4, SOCS-4, Suppressor of Cytokine Signaling 7, SOCS-7, Socs7)
Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-4 is the least studied member of the SOCS family. It is 440aa long and has 90% identical sequence in SH2 region with SOCS-5. Recent studies revealed that SOCS-4 is involved in degradation of epidermal growth factor receptor (EGFR). Specifically, SOCS-4 binds to pY1092 EGFR site.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the N-terminus of human SOCS-4.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Recognizes human SOCS-4. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hilton D. J. et al. (1998) Biochemistry 195: 114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15 (11): 1493-1504. 4. Nicholson S.E. et al. (2005) PNAS 102 (7): 2328-2333 5. Kario E. et al. (2005) J. Biol. Chem. 280 (5): 7038-7048.