		Technical Data
S1040-04J	SOCS4 (Suppressor Of Cytokine Signaling 4, SOCS-4, Suppressor of Cytokine Signaling 7, SOCS-7, SOCS7)

Description:
Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-4 is the least studied member of the SOCS family. It is 440aa long and has 90% identical sequence in SH2 region with SOCS-5. Recent studies revealed that SOCS-4 is involved in degradation of epidermal growth factor receptor (EGFR). Specifically, SOCS-4 binds to pY1092 EGFR site.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-SKVRVLRIDAPEQQ, from SOCS4, at C-terminal (NP_543143.1, NP_955453.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes SOCS4. Species sequence homology: Bovine, human and porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kario E, Marmor MD, Adamsky K, Citri A, Amit I, Amariglio N, Rechavi G, Yarden Y. Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factor receptor signaling. J Biol Chem. 2005 Feb 25;280(8):7038-48.