Technical Data
SOCS 5 (Suppressor of Cytokine Signaling 5, SOCS5, SOCS-5, Cytokine-inducible SH2-containing Protein 5, CISH5, Cytokine-inducible SH2 Protein 6, CIS6, CIS-6, CISH6, KIAA0671)
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR. Involved in the regulation of T-helper cell differentiation by inhibiting of the IL4 signaling pathway which promotes differentiation into the Th2 phenotype. Can also partially inhibit IL6 and LIF signaling.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Western Blot: 0.3-1ug/ml, observed in lysates of cell line Molt4 on ~65kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to DKVGKMWNNFK-C, from human SOCS5, at N-terminal (NP_054730.1).
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Recognizes human SOCS5. Species sequence homology: Bovine, mouse, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kuhad A, Bishnoi M, Tiwari V, Chopra K. Suppression of NF-kappabeta signaling pathway by tocotrienol can prevent diabetes associated cognitive deficits. Pharmacol Biochem Behav. 2009 Apr;92(2):251-9.