Technical Data
Spectrin, alpha (Spectrin alpha Chain, Spectrin alpha Chain Brain, Spectrin Non-erythroid alpha Chain, Alpha Fodrin, Alpha II Spectrin, FLJ44613, Fodrin alpha Chain, Non-erythrocytic Spectrin alpha, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)
SPTBN1 is a major constituent of the erythrocyte skeleton, accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol. While the simplest form of SPTBN1 in solution is an alpha-beta heterodimer, SPTBN1 can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of SPTBN1-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
This antibody maps to a region between residue 2150 and 2200 of human Spectrin Non-Erythroid Beta 1 using the numbering given in entry NP_003119.2 (GeneID 6711).
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.