Technical Data
Spectrin, alpha (Spectrin Non-erythroid alpha Chain, Alpha Fodrin, Alpha II Spectrin, FLJ44613, Spectrin alpha Non-erythrocytic 1, SPTAN1, SPTA2)
Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that has been implicated in the establishment of specialized membrane- cytoskeletal domains in differentiating cells (2-3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells, or treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of the cleavage are amino-terminal (135kDa) and carboxy-terminal (150kDa and 120kDa) fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis, and are valuable in the evaluation fo Sicca- and Sjögren's syndrome (5-6).

Suitable for use in Immunocytochemistry, Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-2000
Immunocytochemistry: 1:100-250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A synthetic peptide corresponding to residues in human Alpha Fodrin.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human Alpha Fodrin. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leto TL, et al. Mol Cell Biol. 8(1):1-9, 1988. 2. Simonovic M, et al. J Biol Chem. 281(45):34333-40, 2006. 3. DH Giebelhaus, et al. The Journal of Cell Biology 105:843-853,1987. 4. Martin SJ, et al. J Biol Chem. 270(12):6425-8, 1995. 5. Ulbricht K U, et al. Autoimmunity Reviews 2(2):109-113, 2003
6. Kahaly, G. J., et al. Clinical & Experimental Immunology 140:166-172(7), 2005.