Technical Data
S5379-98
Spectrin, alpha, Erythrocytic 1 (Erythroid alpha-Spectrin, Spectrin alpha Chain Erythrocyte, SPTA, SPTA1, Elliptocytosis 2, EL2, HPP, HS3, SPH3)
Description:
Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1AF10Ascites
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Ghost proteins of human red blood cells
Purity:
Ascites
Form
Supplied as a liquid in PBS, 1% BSA, 0.1 % sodium azide.
Specificity:
Recognizes the 240kD human erythroid a-spectrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tani T. et al. (1996) Exp. Hematol. 24: 24, 158-168.