Technical Data
S5381-03A
Spectrin (Alpha I Spectrin, Alpha-Spec, Beta I Spectrin, Beta Spectrin, Beta-Spec, Elliptocytosis 2, Elliptocytosis-2, EL2, Erythrocyte alpha Spectrin, Erythroid Alpha Spectrin, HSpTB1, Membrane Cytoskeletal Protein, Spec B, Spectrin alpha Chain, Spec A,
Description:
Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: Extract of human erythrocyte ghosts.

Cellular Localization: Cytoplasmic surface of the cell membrane

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19E721Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Full length native Human erythroid spectrin was used as the immunogen (purified).
Purity:
Ascites
Form
0.1% Sodium Azide Raw Ascites
Specificity:
Monclonal anti-Spectrin (alpha and beta) localizes the 220kD (beta) and 240kD (alpha) bands of human erythrocyte spectrin as well as a number of polypeptides in the region of 170, 150, 85, and 45kD. It is likely these lower molecular weight polypeptides may have an epitope shared by both the alpha and beta subunits of spectrin. Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.