Technical Data
S5381-03C
Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA)
Description:
Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100 -1:500
ELISA: 1:105-1:106
Western Blot: 1:102-1:103
Immunohistochemistry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19L188Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant alpha I spectrin (erythroid spectrin) SH3 domain
Purity:
Purified by ammonium sulfate precipitation and dialysis.
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes alpha I spectrin SH3 domain. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. Species Crossreactivity: human, rat, mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.