Technical Data
S5381-03C
Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA)
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Technical Data |
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S5381-03C |
Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA) |
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Description: Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100 -1:500 ELISA: 1:105-1:106 Western Blot: 1:102-1:103 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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