		Technical Data
S5381-25	Spectrin, beta, Erythrocytic (Erythroid beta-Spectrin, Spectrin beta Chain Erythrocyte, SPTB, Beta-I Spectrin, SPTB1, EL3, HS2, HSPTB1, SPH2)

Description:
Erythroid spectrins, some other proteins of erythroid cytoskeleton, and the transmembrane protein band 3 are highly specific for erythrocytes and their progenitors. They are more reliable markers for erythroid differentiation than Glycophorin A, the commonly used marker for erythroid differentiation, because Glycophorin A is expressed also in many cell lines otherwise exhibiting mainly megakaryotic charasteristics.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	DB2	Ascites

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Ghost proteins of human red blood cells

Purity:

Ascites

Form

Supplied as a liquid in PBS, 0.1 % sodium azide, 1% BSA.

Specificity:

Recognizes the 220kD human erythroid b-spectrin. Present in the membranes of human skeletal muscle cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Auranen M. et al. (2000) Neuromusc. Disord. 10, 16-23. 2. Tani T. et al. (1996) Exp. Hematol. 24: 24, 158-168.