Technical Data
S5390
Spermine, Conjugated
Description:
Spermine, a polyamine, is a positively charged compound found in living cells and can cause conformational changes in DNA via charge and solubility mechanisms which can affect gene expression cell differentiation. Spermine may be linked to the development of cancer.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.


Recommended Dilution:
ELISA: 1:2000-1:5000
Immunohistochemistry: 1:500- 1:1000
Western Blot: 1:1000- 1:2000
Optimal dilutions to be determined by the researcher.

Specificity:
Using a conjugate Spermine- Glutaric Anhydride-Protein, antibody specificity was performed by competition ELISA with the following compounds:

Compound Cross-reactivity ratio
Spermine-GA-BSA 1
Spermidine-GA-BSA 1:3
Diamino-Pentane-G-BSA 1:700
Ornithine-GA-BSA 1:500

G = Glutaraldehyde, GA = Glutaric Anhydride

Control Peptide: S5390-01

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic Spermine-Glutaric Anhydride-BSA.
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2, 40% glycerol. Preservative free.
Specificity:
Recognizes protein-conjugated Spermine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.