Technical Data
S5620-01B
Sprouty 1 (Spry 1)
Description:
Sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells. Four mammalian Sprouty homologues (Sprouty-1–4) have been identified. One study showed that overexpressed Sprouty-1 and -2 inhibits fibroblast growth factor– and vascular endothelial growth factor–induced proliferation and differentiation by repressing
pathways leading to MAP kinase activation.1 Sprouty proteins are also endogenous inhibitors of the Ras/MAP kinase pathway that play an important role in the remodeling of branching tissues, such as in development of the lung, kidney tubules, vascular system, and breast ducts.
A recent study shows that Sprouty-1 and –2 may have tumor suppressing activity in the breast, and found that both proteins are consistently down-regulated in breast carcinomas.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of mouse and rat Sprouty-1
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide.
Specificity:
Recognizes mouse Sprouty-1 at ~34kD. A band of unknown origin was observed at ~120kD. Reactivity has been confirmed with two day-old mouse kidney homogenates. Does not recognize rat Sprouty-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Impagnatiello MA, et al. J Cell Biol 152(5):1087-1098, 2001.
2. Lo TL, et al. Cancer Res 64(17):6127-6136, 2004.