Technical Data
S5620-03
Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039)
Description:
Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling. Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras. Sprouty expression is induced by the same pathways that it down-regulates, suggesting it plays a role in an auto-feedback loop. Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-1ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
F4209: 3T3/A31 lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 10ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species Crossreactivity: Human and mouse. Crossreactivity with rat predicted, based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hacohen, N., et al., Cell 92: 253-263 (1998). 2. Hanafusa, H., et al., Nature Cell Biol. 4: 850-858 (2002). 3. Wong, E.S., et al., J. Biol. Chem. 276: 5866-5875 (2001). 4. Yusoff, P., et al., J. Biol. Chem. 277: 3195-3201 (2002).