Technical Data
S5620-25C
SPRY4, CT (Protein Sprouty Homolog 4, Spry-4)
Description:
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human SPRY4 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human SPRY4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Tsai CF, et al. (2008) J Proteome Res. 2.Guo A, et al. (2008) Proc Natl Acad Sci USA 105, 692-7. 3.Rikova K, et al. (2007) Cell 131, 1190-203. 4.Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci USA 104, 5860-5. 5.Tsumura Y, et al. (2005) Biochem J 387, 627-37.