		Technical Data
S6600-65A	SSA1 (Sjogren Syndrome Antigen A1, SSA1 Sjogren Syndrome Antigen A1 (52kD Ribonucleoprotein Autoantigen SS-A/Ro, Ro/SS-A, Ro(SS-A), RO52, RNF81, Sjogren Syndrome Type A Antigen, SS-A, TRIM21, Tripartite Motif Protein TRIM21)

Description:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is part of the RoSSA ribonucleoprotein which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. The function of the RoSSA particle has not been determined. Two alternatively spliced transcript variants for this gene have been described; however, the full length nature of one variant has not been determined.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000
Western Blot: 1ug/ml detected bands at ~38kD and 25kD in human brain lysates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to CPLNIGSQGSTDY, aa463-475, from the C-Terminus of human SSA1 (Accession #NP_003132). Cellular Localization: Cytoplasmic, nuclear.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human SSA1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Chan EK et al. 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart. J Exp Med 182:983-92 (1995). Itoh K et al. Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes. J Clin Invest 87:177-86 (1991).