		Technical Data
S7965-38B	Staphylococcus aureus alpha Hemolysin

Description:
Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		4°C (-20°C Glycerol)	Blue Ice		Sheep

Concentration:

~1mg/ml

Immunogen:

Staphylococcal alpha Hemolysin.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute in 1ml 40-50% glycerol, dH2O.

Specificity:

Recognizes Staphylococcal alpha Hemolysin. Minimal reactivity with staphylococcal enterotoxins A through E and TSST.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.