Technical Data
S7969-05A
STAT 1 (Signal Transducer and Activator of Transcription 1)
Description:
Stat1, while activated in response to a large number of ligands (1), appears to be essential for responsiveness to IFN-alpha and IFN-gamma (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding (4). Stat1 has two isoforms, Stat1alpha (91kD) and the splice variant Stat1beta/Stat2 (84kD). In most cells, both isoforms are activated by IFN-alpha, but only Stat1alpha is activated by IFN-gamma. Stat1 has been found to be inappropriately activated in many tumors (5).

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (KLH coupled) derived from the sequence of human Stat1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Recognizes endogenous levels of total human Stat1 protein. Detects Stat1alpha (91kD) and Stat1beta (84kD). Species Crossreactivity: mouse and rat. Species sequence homology: bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Heim, M.H. (1999) J. Recept. Signal. Transduct. Res. 19, 75-120. 2. Durbin, J.E. et al. (1996) Cell 84, 443-450. 3. Meraz, M.A. et al. (1996) Cell 84, 431-442. 4. Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227. 5. Frank, D.A. (1999) Mol. Med. 5, 432-456. 6. Peterson, W.M. et al. (2000) J. Neurosci. 20, 4081-4090. 7. Suzuki, K. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 2285-2290. 8. Chen, G. et al. (2001) J. Biol. Chem. 276, 766-772. 9. Hutt, J.A. et al. (2000) J. Biol. Chem. 275, 29123-29131.10. Mahboubi, K. and Pober, J.S. (2002) J. Biol. Chem. 277, 8012-8021.