Technical Data
S7969-06Q
STAT 1 (Signal Transducer and Activator of Transcription 1-alpha/beta, Transcription Factor ISGF-3 Components p91/p84)
Description:
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. STAT1 is involved in the TNFR1-TRADD signaling complex. In Hela cells STAT1 acts as a TNFR1-signaling molecule to suppress NF-kB activation. It has also been shown that its activation slows growth and promotes apoptosis. Human STAT1 gene is mapped to chromosome 2q32.2.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:50-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
COLO205 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic non-phosphopeptide corresponding to human STAT1 around the phosphorylation site of tyrosine 701(T-G-YP-I-K).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human STAT1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jacqueline, M. et al. J.Biol. Chem. 273:31408-31416 (1998). 2. Anastasis, S. et al. J. Biol. Chem. 275:10002-10008 (2000). 3. Wang, Y. et al. Mol. Cell. Biol. 20:4505-4512 (2000).