		Technical Data
S7971-01M	STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)

Description:
Domain Information: SH2 domain; STAT protein, DNA binding domain; STAT protein, all-alpha domain; STAT protein, protein interaction domain.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA (Peptide): 1:128,000 (antibody detection limit dilution)
Western Blot: 1-3ug/ml. A band at ~90kD is observed in NIH3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour; detection by ECL. Predicted MW of 88kD according to NP_003141.2.
Immunohistochemistry: 10ug/ml (overnight incubation). Stains mouse thymic medulla and not the cortex.
Optimal dilutions to be determined by the researcher.

Peptide Blocking:
S7971-01M-P

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Peptide corresponding to DMELTSECATSPM from the C-terminus of the human protein sequence. (Accession # NP_003141.2 and NP_644805.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human STAT 3. Species Crossreactivity: mouse. Species sequence homology: rat, canine, porcine and bovine. Sequence homology: human isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr. , Stat3 as an oncogene., Cell. 1999 Aug 6;98(3):295-303. 2. Erratum in: Cell 1999 Oct 15;99(2):239.