Technical Data
S7971-01M
STAT3 (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF, FLJ20882, MGC16063)
Description:
Domain Information: SH2 domain; STAT protein, DNA binding domain; STAT protein, all-alpha domain; STAT protein, protein interaction domain.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA (Peptide): 1:128,000 (antibody detection limit dilution)
Western Blot: 1-3ug/ml. A band at ~90kD is observed in NIH3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour; detection by ECL. Predicted MW of 88kD according to NP_003141.2.
Immunohistochemistry: 10ug/ml (overnight incubation). Stains mouse thymic medulla and not the cortex.
Optimal dilutions to be determined by the researcher.

Peptide Blocking:
S7971-01M-P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide corresponding to DMELTSECATSPM from the C-terminus of the human protein sequence. (Accession # NP_003141.2 and NP_644805.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human STAT 3. Species Crossreactivity: mouse. Species sequence homology: rat, canine, porcine and bovine. Sequence homology: human isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE Jr. , Stat3 as an oncogene., Cell. 1999 Aug 6;98(3):295-303. 2. Erratum in: Cell 1999 Oct 15;99(2):239.