Technical Data
S7973-01H
Stem Cell Factor, Recombinant, Human, aa26-189 (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Source:
Recombinant corresponding to aa26-189 of human Stem Cell Factor expressed in E.coli.

AA Sequence:
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.5kD
Source: Human, Recombinant, E. coli
Purity: 95% by SDS-PAGE. Purified by using conventional chromatography techniques.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Zhang, Z., et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97: 7732 (2000). 2. Okada, S., et al., Nippon Rinsho 50: 1872 (1992).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.