Technical Data
S7973-02
Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
Recombinant Human SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.

Sequence:
First five N-terminal amino acids: Met-Glu-Gly-Ile-Cys.

Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 10e5 IU/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C..Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.4kD
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Form: Supplied as a lyophilized powder from 0.02% sodium bicarbonate. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of >0.1mg/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ex vivo manipulation of umbilical cord blood-derived hematopoietic stem/progenitor cells with recombinant human stem cell factor can up-regulate levels of homing-essential molecules to increase their transmigratory potential. Zheng Y, Watanabe N, Igura K, Takaue Y, Exp Hematol 20 03 Dec;31(12):1237-46 2. Moon TC, Lee E, Murakami M, Min HK, Mol Cells 2003 Oct 31;16(2):154-60 3. Han J, Yan X, Zhi X, Protein Expr Purif 2003 Oct;31(2):311-7 4. [Expression of recombinant human stem cell factor in insect cells] Zhang D, Qin J, Zhu J, Wei Sheng Wu Xue Bao 2000 Feb;40(1):44-9 5. [The expression study of human stem cell factor (hSCF) in E. coli] Mao H, Zhao J, Di X, Zhongguo Yi Xue Ke Xue Yuan Xue Bao 1998 Aug;20(4):279-84 6. Zhang Z, Zhang R, Schlessinger J, Proc Natl Acad Sci U S A 2000 Jul 5;97(14):7732-7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.