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Technical Data |
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S7973-04 |
Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF) |
2ug 10ug 100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant Human SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. Endotoxin:<0.1EU/ug as per LAL Biological Activity: Recombinant human SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <1.7ng/ml. AA Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVALPPVA Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 18.5kD |
Source: Recombinant, human, E. coli Purity: ~ 98% as determined by RP-HPLC or Reducing and non-reducing SDS-PAGE. Form: Supplied as a lyophilized powder from 10mM acetic acid. Reconstitute with sterile ddH2O, 0.1% HSA or BSA, to 0.1mg/ml, which can be further diluted into other aqueous solutions. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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