Technical Data
S7973-11
Stem Cell Factor, Recombinant, Mouse (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Murine SCF is an 18.3kD protein containing 164 amino acid residues (secreted soluble form).

AA Sequence :
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Biological Activity:
The ED50, determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is
10ng/ml corresponding to a specific activity of 1x10e5u/mg. For most in vitro applications, SCF exerts its biological activity in the range of 1-100ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA

Molecular Weight:
18.3kD
Source: E. coli
Purity: Highly purified ( 98% by SDS-PAGE and HPLC analyses).
Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 6.5. Reconstitute with sterile dH2O to a concentration of 0.1-1.0mg/ml with carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.