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Technical Data |
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S7973-11A |
Stem Cell Factor, Recombinant, Mouse (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant mouse Stem Cell Factor is a single, non-glycosylated, polypeptide chain containing 164aa and having a molecular weight of 18.3kD. The sequence of the first five N-terminal amino acids was determined to be Met-Lys-Glu-Ile-Cys. Contains less than 1% dimers and aggregates. Source: E. coli Molecular Weight: ~18.3kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is <10ng/ml, corresponding to a specific activity of 1x10e5IU/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with ddH2O. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 18.3kD |
Source: E. coli Purity: 98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE). Endotoxin: 0.1ng/ug Form: Supplied as a lyophilized powder from 0.02% sodium bicarbonate. Reconstitute with sterile deionized water to a concentration 100ug/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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