Technical Data
S7973-12
Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
2ug
10ug
10ug
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Technical Data |
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S7973-12 |
Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Recombinant murine SCF is a single, non-glycosylated polypeptide chain containing 165 amino acids. Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA. Biological Activity: Recombinant Murine SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of Human TF-1 cell line is <10ng/ml, corresponding to a Specific Activity of 1x10e5 IU/mg. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of SCF as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 1.8309kD |
Source: Murine, recombinant protein expressed in E. coli Purity: Chromatographically purified ( 98% by SEC-HPLC, SDS-PAGE). Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder from 0.02% NaHCO3, pH 6.5. Reconstitution: Reconstitute with sterile ddH2O, 0.1% HSA or BSA to a concentration >100ug/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Ferret-Bernard, S., et al., Immunol. Lett. 91(2-3): 221-7 (2004, Feb 15). 2. Sykes, D.B., et al., Mol. Cell. Biol. 24(3): 1256-69 (2004, Feb). 3. Hartung, G., et al., Bone Marrow Transplant 32(1): 49-56 (2003, Jul). 4. Ohmura, M., et al., Biol. Reprod. 68(6): 2304-13 (2003, Jun). 5. Simpson, K., et al., Lab. Invest. 83(2): 199-206 (2003, Feb). 6. Metcalf, D., et al., Stem Cells 20(5): 460-9 (2002).
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