Technical Data
S7973-12A
Stem Cell Factor, Recombinant, Mouse (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
2ug
10ug
100ug
Molecular Biology Storage: -20°CShipping: Blue Ice
Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance and surivial of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant murine SCF produced in E. coli is a single, non-glycosylated polypeptide chain containing 164aa and having a molecular mass of 18.3kD (P20826).

Biological Activity:
The activity is determined by the dose-dependent proliferation of human TF-1 cells and is typically <10ng/ml.

Amino Acid Sequence:
KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.3kD
Source: Recombinant, murine, E. coli
Purity: ~97% as determined Reducing and non-reducing SDS-PAGE. Endotoxin: 1EU/ug (LAL).
Form: Supplied as a lyophilized powder from 10mM sodium phosphate buffer, pH 6.5. Reconstitute with sterile ddH2O to 0.1mg/ml. For long term storage, add a carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Horváth, V.J., et al., Gastroenterology 130(3): 759-770 (2006). 2. Kimura, S., et al., J. Invest. Dermatol. 124(1): 229-234 (2005). 3. Ferret-Bernard, S., et al., Immunol. Lett. 91(2-3): 221-227 (2004). 4. Sykes, D.B. & Kamps, M.P., Mol. Cell. Biol. 24(3): 1256-1269 (2004). 5. Hartung, G., et al., Bone Marrow Transplant. 32(1): 49-56 (2003). 6. Ohmura, M., et al., Biol. Reprod. 68(6): 2304-2313 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.