Technical Data
S7973-13
Stem Cell Factor, Recombinant, Mouse (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)
100ug
Molecular Biology Storage: -20CShipping: Dry Ice
Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant mouse SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight:
18kD (165aa)

Sequences:
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18kD
Source: Recombinant, Murine E coli
Purity: 95% by SDS PAGE.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4. 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732.
Okada S , et al. (1992). Nippon Rinsho , 50, 1872.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.