Technical Data
S7973-30
Stem Cell Growth Factor beta, Recombinant, Human (SCGFb)
2ug
10ug
10ug
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Technical Data |
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S7973-30 |
Stem Cell Growth Factor beta, Recombinant, Human (SCGFb) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Stem Cell Growth Factor alpha and beta are hematopoietic growth factors that exert their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGFb is a 25kD polypeptide containing 227 amino acid residues. Endotoxin: 0.1ng/ug (1EU/ug). Reconstitution: Reconstitute with sterile ddH2O to 0.1-1.0mg/ml. Add buffer containing carrier protein for long term storage. Biological Activity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 7ng/ml. AA Sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Add carrier protein and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA Molecular Weight: 25.0kD |
Source: E. coli Purity: 98% by SDS-PAGE and HPLC. Form: Supplied as a lyophilized powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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