Technical Data
S7975-15A
Stromal Cell Derived Factor 1alpha (SDF1 alpha, SDF1a, SDF-1a, Chemokine (C-X-C Motif) Ligand 12, CXCL12, hIRH, Pre-B cell Growth-stimulating Factor, PBSF, SCYB12, TLSF-a, TPAR1)
Description:
SDF-1a (stromal cell-derived factor-a), also known as pre-B-cell growth-stimulating factor (PBSF), is a 70-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli-expressed mouse SDF-1a
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes mouse SDF-1a. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tashiro, K., et al., Science 261: 600-603 (1993). 2. Nagasawa, T., et al., Proc. Natl. Acad. Sci. USA 91: 2305-2309 (1994). 3. Nagasawa, T., et al., Nature 328: 635-638 (1996). 4. Bleul, C.C., et al., J. Exp. Med. 184: 1101-1109 (1996). 5. Bleul, C.C., et al., Nature 382: 829-833 (1996). 6. Oberlin, E., et al., Nature 382: 833-835 (1996).